Comments on: The Realities of Resolution http://photo.blogoverflow.com/2012/06/the-realities-of-resolution/ The Photography Stack Exchange Blog Thu, 01 Dec 2016 09:08:25 +0000 hourly 1 https://wordpress.org/?v=4.5.6 By: Lukas http://photo.blogoverflow.com/2012/06/the-realities-of-resolution/#comment-300226 Tue, 27 Jan 2015 19:31:58 +0000 http://photo.blogoverflow.com/?p=542#comment-300226 Nice article, for curious I will add that nowadays you can go around light diffraction limit in optical system if you do fluorescence imaging of your object, you shine strong (laser) light on it and detect another light coming out from it with boosted CCD sensors for example. If you hook up such sensor to a microscope with a fancy lens (objective) you can even resolve neighboring objects within your diffraction limited light spot! If you focus at one such object (another one has to be turned off for now!) you will see its regular diffraction limited image on the sensor, i.e. again light spot spread over a few pixels in each dimension. However, if you fit light intensity of pixels holding this spot image with a special mathematical function you will get a center of the object with accuracy of a few nanometers only. After you imaged first object (that is turned off now) you can turn on the second object and do the same – this effectively means you can resolve two such objects if they are only tens of nanometers apart. The trick here is you need to be able to measure only one object at a time, one by one, you read all the light from first excited object (fluorescence light finally runs out) and then you go onto another object, etc… After you read fluorescence light from all the objects (one at a time) you can overlay and render the final image with all the objects resolved at just tens of nanometer resolution. This year’s Nobel Prize in Chemistry was awarded for this technique, also called supperresolution or PALM/STORM.

]]> By: yoyo http://photo.blogoverflow.com/2012/06/the-realities-of-resolution/#comment-3996 Mon, 24 Dec 2012 17:09:08 +0000 http://photo.blogoverflow.com/?p=542#comment-3996 Thank you. Very nice and informative. Really enjoyed it, especially he realworld examples of lenses and the graph.

🙂

]]> By: jrista http://photo.blogoverflow.com/2012/06/the-realities-of-resolution/#comment-1537 Fri, 31 Aug 2012 01:50:10 +0000 http://photo.blogoverflow.com/?p=542#comment-1537 Glad you enjoyed it. 🙂 I try to be as informative as I can be. (Sorry for the late reply…I’ve been putting more of my time into photography than this site lately, so I’m way behind.)

]]> By: Matthias Boettrich http://photo.blogoverflow.com/2012/06/the-realities-of-resolution/#comment-1105 Fri, 13 Jul 2012 21:03:26 +0000 http://photo.blogoverflow.com/?p=542#comment-1105 I came to this article because I was interested in diffraction and determining the optimal amperture for a sensor-lens combo. I also enjoyed reading the rest of your well written and informative essay. Thank you!

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